Rowles Lagoon is an ephemeral freshwater wetland located in the Goldfields Region of Western Australia, approximately 73–103 kilometres north of Coolgardie and around 103 kilometres northwest of Kalgoorlie. It is the largest freshwater lake in the Coolgardie bioregion and is listed on Australia's Register of Significant Wetlands. The lagoon sits within Rowles Lagoon Conservation Park, which is managed for nature conservation and carries no park entry fee.

The water level varies dramatically with the seasons and rainfall. During drought periods the lagoon can dry out completely, while heavy rain years can fill it to overflowing. Paddling is only feasible when water levels are sufficiently high, so checking conditions well in advance of any trip is essential. When water is present, canoeing and kayaking are permitted activities. Motorised vessels and water skiing are explicitly prohibited to protect the wildlife and tranquility of the reserve. The Parks and Wildlife Service recommends reading safety information about paddling and kayaking before visiting, and travelling with a personal location beacon (PLB) is advised given the remote location.

The lagoon is surrounded by fringing tea tree thicket, which provides natural cover for observing wildlife. Forty-one species of waterbirds have been recorded here, including eight species protected under international treaties. Commonly sighted species include Black Swan, Australian Shelduck, and Hoary-headed Grebe. The rare Freckled Duck, one of the world's rarest waterfowl, has been recorded breeding at the lagoon on two occasions. The surrounding vegetation also supports a range of bushbirds.

Access to the lagoon from Kalgoorlie involves travelling northwest along the Goldfields Highway, turning onto the Ora Banda–Broad Arrow Road, then southwest on the Ora Banda Carbine Road, followed by the Coolgardie North Road. Portions of the access road are unsealed. Facilities at the campground include free camping sites, BBQs, and toilets. Campfires are not permitted between 1 November and 30 April. Dogs and other pets are not allowed within the park. The Kalgoorlie Parks and Wildlife Office can be contacted at (08) 9080 5555 for current park information.