Arroyo Pergamino is a watercourse in Buenos Aires Province, Argentina, with a total length of approximately 100 kilometers. It originates in the Bañados del Juncal, near the border with Santa Fe Province, and flows in a southeasterly direction until it empties into the Río Arrecifes. The arroyo's flow is supplemented by smaller, temporary tributaries along its banks. The waterway passes through the city of Pergamino, which shares its name. Access points and specific facilities like boat ramps are available. The overall character of the arroyo is shaped by the surrounding Pampas landscape. Water levels can fluctuate significantly, with a normal height at the Puente Merced monitoring point being around 1.50 meters, while an alert level is triggered at 4.50 meters, indicating a substantial rise that could present hazards. This variability suggests paddling conditions are highly dependent on recent rainfall. The arroyo is a known destination for anglers, indicating the presence of fish populations. A trail provides access to the arroyo for viewing.