The Flora River flows through the Katherine Region of the Northern Territory, located roughly 70 kilometres west of Katherine. The river is entirely spring-fed, which gives the water a distinctive aqua colour unlike typical Territory waterways. It flows through Flora River Nature Park before eventually joining the Katherine River, at which point it becomes the Daly River.

The primary put-in is at Kathleen Falls, which serves as the park campground. Access to this point requires travelling approximately 20 kilometres of rough bulldust track, making a high-clearance four-wheel-drive vehicle essential. A car shuffle is necessary for through-trips, which adds considerable logistical complexity, particularly for larger groups.

A multi-day route paddling from Kathleen Falls to the junction with the Katherine River has been completed as a three-day trip with two nights of camping. The river features tufa dams — natural calcium carbonate formations — that require paddlers to exit their boats and either portage or carefully lower craft over the drops. The waterfall at one campsite along the route also required lowering canoes. Exit points along the river are limited, so paddlers should plan accordingly and carry sufficient supplies between known stopping points.

The Kathleen Falls area provides a notable backdrop at the start of the trip, with multiple waterfalls visible from the water. Wildlife in the park includes barramundi, and the area is noted for its natural character. Walking tracks exist within the park, though conditions can be overgrown. Camping facilities are available at the park campground near the put-in.