Jennejohn Lake is located in the Northwest Territories near Yellowknife, and serves as a central highlight of the Jennejohn canoe route along the Ingraham Trail. The lake is notably clear and deep, with a reputation for good fishing, particularly for lake char. The surrounding area supports a diverse bird population including Arctic terns, ducks, loons, and both bald and golden eagles, which can often be spotted soaring overhead or perched in solitary trees. Eagles are known to nest on rocky cliff faces in the area.

The Jennejohn route is classified as a five-day wilderness trip for advanced paddlers, covering approximately 60 km in total. The route begins at Reid Lake Territorial Campground, located at kilometre 59 on the Ingraham Trail, which provides a canoe launch, 65 non-powered campsites, tent sites, showers, a kitchen shelter, firewood for sale, a boat launch, and a playground. From Reid Lake, the route follows a chain of lakes before reaching Jennejohn Lake, then continues onward through Mason Lake (approximately 10 km long, with camping and fishing available along the south shoreline) and eventually arrives at Dettah on Great Slave Lake. Paddlers can either be picked up at Dettah or continue an additional 7 km along Yellowknife Bay into Yellowknife.

The route involves multiple portages of varying lengths. Notable carries include a 500 m portage, a paddle across a small lake, and a 700 m portage leading to Mason Lake, as well as a 1 km portage to Duck Lake and a 350 m portage into Great Slave Lake from the southwest bay of Duck Lake. Many of the portages beyond Mason Lake follow well-traveled winter trails. A narrow west arm channel of approximately 8 km includes one short portage in the middle.

Given the advanced classification and multi-day wilderness nature of the route, paddlers should be well-prepared for remote travel. The Robertson Shaft of Con Mine, identifiable by its bright red top, is visible from Duck Lake near the end of the route, providing a notable landmark as paddlers approach the Yellowknife area.