Lough Leane is the largest of the three Lakes of Killarney, covering approximately 4,700 acres (19 square kilometers), and sits within Killarney National Park in County Kerry on the southwest coast of Ireland. It is the northernmost and lowest of the three lakes, connected to Muckross Lake (the Middle Lake) by a short river, which in turn links via the Long Range channel to the Upper Lake. The surrounding landscape is dominated by Macgillycuddy's Reeks, the highest mountain range in Ireland, providing a dramatic backdrop throughout any paddle.

The primary access point for paddlers is Ross Castle, where launches typically begin from the shore near Reen Pier. From here, paddlers can reach Innisfallen Island, located roughly one mile (1.6 km) offshore. The island, covering 21 acres, holds the ruins of Innisfallen Abbey, originally constructed in the 6th to 7th century and a significant monastic site during the Middle Ages — the name Lough Leane itself is said to mean "Lake of Learning" in reference to this history. Other points of interest accessible by water include the Bronze Age copper mines of Ross Island, Glena, and O'Sullivan's Cascade.

A popular multi-lake route begins at Lord Brandon's Cottage on the Upper Lake, paddling down the Long Range river, passing under Old Weir Bridge at the Meeting of the Waters, continuing past Dinis Cottage and through Muckross Lake, then under Brickeen Bridge and into Lough Leane. This route traverses all three lakes and includes at least one section of mild rapids. Paddlers finishing at Ross Castle can optionally extend the trip with a paddle out to Innisfallen Island.

Wind is a notable consideration on Lough Leane. The lake's size means that when wind picks up, conditions can become choppy and paddling against it requires significant effort. Wetsuits are recommended given the cold water and the likelihood of rain, particularly outside of summer months. Guided tours operating on the lake provide wetsuits and equipment and cater to all experience levels. White-tailed Sea Eagles have been spotted in the area, adding to the wildlife interest for paddlers on the water.