Dysart Harbour sits on the Fife coast in what is now a north-eastern suburb of Kirkcaldy, Scotland. The harbour was partly carved out of coal that once outcropped along the shoreline, and today shelters a variety of pleasure craft.

Its north-western edge is bounded by a high natural stone wall, along the top of which runs the Sailors' Walk — a path connecting the town to the outer harbour via a flight of stairs.

The harbour is a popular stop on the Fife Coastal Path and is flanked by the historic Pan Ha' houses and the remains of St Serf's Church.

The Harbourmaster's House, now headquarters of the Fife Countryside Trust, overlooks the water and contains a café with views across the harbour.