Arroyo Yabotí Miní originates in the northeastern extreme of the Sierra de Misiones and flows from north to south, running along the eastern edge of Parque Provincial Esmeralda, which forms the core zone of the Reserva de Biosfera Yabotí. Where it meets the Arroyo Yabotí Guazú, the two streams combine to form the Arroyo Yabotí, which in turn flows into the Río Uruguay a short distance downstream from the Saltos del Moconá.

A documented expedition in February 2018 navigated approximately 9 km along the Arroyo Yabotí Miní and a further 22 km along the Yabotí, beginning at the margins of the Guaraní community of Tucuaruchú and ending at the bridge marking the entrance to Parque Provincial Moconá. This route passed through areas of the Parque Provincial Esmeralda that had not previously been explored, suggesting the corridor is remote and sees very little traffic. The expedition was conducted over two days, giving an indication of the time commitment involved for this stretch.

The surrounding landscape is characterized by primary rainforest, rocky walls, and tributary streams — features consistent with the dense Atlantic Forest of Misiones. The area falls within a protected biosphere reserve, and the expedition's scientific purpose involved collecting ferns and lycophytes, reflecting the botanical richness of the riparian environment.

Detailed information on access logistics, hazards, water levels, facilities, and regulations is limited. Paddlers considering this route should seek current guidance from local authorities or park administration for the Reserva de Biosfera Yabotí and Parque Provincial Esmeralda, as the waterway borders protected core zones where specific rules may apply.