The Tolomato River is a tidal saltwater river and part of the Atlantic Intracoastal Waterway in northeast Florida, offering paddlers an undeveloped natural environment. Several public launches provide access. The Nocatee Paddle Launch, also called Nocatee Landing, is a primary access point located off Tolomato Road or Nocatee Landing Trail in Ponte Vedra Beach. It features a gravel road leading to a quiet landing with free parking, solar-powered restrooms, an open-air pavilion, and bike racks, and is open from dawn to dusk. Another key launch is at the Guana Dam within the Guana Tolomato Matanzas (GTM) Research Reserve, which has a $3 per vehicle day-use fee, restrooms, ample parking, and two ramps. One ramp provides access to the saltwater Guana River, which flows into the Tolomato River. The river is strongly influenced by tides, and paddlers must plan around them. Currents can be strong, and it is recommended to paddle upstream at the end of an incoming tide and then return downstream as the tide goes out for an easier trip. Launching and returning around high tide is advised, as low tide can make some launches difficult and muddy. The waterway sees boat traffic, which can be heavy on weekends. The overall experience is characterized by tranquil waters and opportunities for wildlife viewing, including alligators, manatees, dolphins, and numerous bird species. A unique seasonal feature is the presence of bioluminescent comb jellies in the Guana Lake and river system during the fall. Paddlers can explore the marshes, paddle around Pine Island, and venture into small tributaries leading into the Deep Creek State Forest. Morning paddles are recommended to beat the summer heat and for more active birdlife. Guided tours are available from various outfitters, some of which offer nighttime bioluminescence experiences.