Sanibel Bayou offers paddlers a diverse experience as part of the Great Calusa Blueway, a 190-mile paddling trail in Southwest Florida. The bayou's distance varies, providing flexibility for different skill levels. Launch points include Sanibel at the Causeway, Tarpon Bay Road, and Bowman's Beach.

Paddlers can explore the nearby Tarpon Bay and Commodore Creek Kayak Trail. Tarpon Bay features an 8-mile loop, while the Commodore Creek Trail extends 2.5 miles through mangrove forests. The area is tidal, so paddlers should be aware of changing water levels.

Wildlife viewing is a highlight, with opportunities to spot dolphins, manatees, ospreys, pelicans, herons, and various wading birds. The J.N. "Ding" Darling National Wildlife Refuge, accessible from Tarpon Bay, is home to 245 bird species and offers additional paddling routes.

Paddlers should be prepared for potentially windy conditions, especially when crossing open water areas like Tarpon Bay. Sticking close to the shoreline on the sheltered side can provide an easier paddling experience. Motor boats may also be present in some areas.

Tarpon Bay Explorers, located within the wildlife refuge, offers kayak rentals, guided tours, and launch facilities for a fee. They provide amenities such as fishing equipment rentals and kayak loading assistance. Other outfitters on the island also offer rental and tour options.

The best time for paddling is when winds are below 10 mph, which is particularly important for novice kayakers. The area is suitable for year-round paddling, with late spring to early fall offering warmer waters.

Paddlers should be mindful of narrow spots in mangrove tunnels and use the numbered trail markers to avoid getting lost. The area's natural beauty, abundant wildlife, and mix of open bays and mangrove-lined waterways create a memorable paddling experience for both beginners and experienced kayakers.