Cibolo Creek originates in the Texas Hill Country near Boerne in Kendall County, flowing approximately 96 miles southeastward before joining the San Antonio River near Helena in Karnes County. The creek derives its name from the Spanish word for buffalo, reflecting the historical presence of American bison that once inhabited the region and were hunted by indigenous peoples along its banks.

The upper reaches in Kendall County feature spring-fed flows through limestone canyons with towering bald cypress, creating scenic pools and occasional small waterfalls. As the creek passes through Bexar County, it forms the eastern boundary of the county and flows through areas like Camp Bullis, where it transitions between perennial and intermittent flows. The middle section through Comal and Guadalupe Counties enters the Edwards Aquifer recharge zone, where water frequently disappears underground through limestone fractures. The lower reaches through Wilson and Karnes Counties return to more consistent flow, meandering through flatter terrain with oak, mesquite, and juniper vegetation before reaching the San Antonio River confluence.

Paddling opportunities exist primarily in the upper watershed near Boerne and in the lower sections through Wilson and Karnes Counties when water levels permit. The San Antonio River Authority maintains portions of the Saspamco Paddling Trail that includes sections of Cibolo Creek, though access and conditions vary significantly with seasonal rainfall. Most paddleable sections are Class I with gentle current, though log jams and low-water crossings require portaging. The creek is not recommended for whitewater enthusiasts, as it lacks significant rapids.

The Cibolo Nature Center in Boerne preserves 100 acres along the creek, providing public access to observe the riparian ecosystem. The creek supports populations of Guadalupe bass, bluegill, channel catfish, and Rio Grande cichlids. Birdwatchers frequently spot great blue herons, green herons, and various songbirds along the wooded corridors.

Historical significance includes Spanish colonial exploration routes and the establishment of missions in the region. Ferdinand Lindheimer collected botanical specimens along the creek in the 1840s, and several historic crossings served as important travel routes during Texas settlement.

Water levels fluctuate dramatically between wet and dry periods, making paddling highly seasonal and dependent on recent rainfall. Spring and early summer typically offer the most reliable flows. The Edwards Aquifer recharge function means that even after significant rain, water may disappear underground in the middle sections. Paddlers should always scout conditions beforehand and be prepared for shallow water, exposed limestone, and potential strainers from fallen trees.