Biloxi Bay is a coastal bay on the Mississippi Gulf Coast, offering a mix of protected and open-water paddling experiences. The bay is bordered by the mainland towns of Biloxi, D'Iberville, and Ocean Springs, with Deer Island providing a natural barrier to the south, separating the bay from the Mississippi Sound. This geography creates generally calm conditions within the bay itself, though the eastern tip of Deer Island is a point where the dynamics of the bay and the Sound can mix, especially on windy days. Paddlers should be aware of tidal influence, particularly when exploring features like the trench on the eastern end of Deer Island, which is accessible at high tide but can become shallow and potentially trap paddlers on a falling tide.

Several public boat ramps provide excellent access to the bay. Key launch points include the Hiller Park Boat Launch in Biloxi, which offers quick access with ample trailer parking, and the Kuhn Street Boat Launch, which provides close access to the barrier islands. In Ocean Springs, paddlers can launch from the Ocean Springs Harbor at the east end of Front Beach or anywhere along Front Beach itself. From there, common routes include exploring the harbor, paddling around the Biloxi Bay Bridge, heading towards Davis Bayou and Marsh Point, or crossing to Deer Island. The paddle from Biloxi Beach to Deer Island is take about ten minutes. On the bay side of Deer Island, a small cove offers a potential break spot, though insects can be prevalent.

The overall character is one of accessible coastal paddling with views of shrimp boats, pelicans, and historic sites. The bay connects to a network of inland waterways, including the Biloxi Back Bay, Mullet Lake, and the Biloxi River Marshes Coastal Preserve to the north, offering extended exploration opportunities. While the bay waters are generally calm, paddlers should be mindful of wind exposure, particularly when venturing near the mouth or into the Mississippi Sound, and always check tide conditions before exploring shallow inlets.